No current evidence for widespread dosage compensation in S. cerevisiae.
نویسندگان
چکیده
Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that this tight correlation between gene copy number and RNA levels is not observed in recently isolated wild Saccharomyces cerevisiae variants. To understand the origins of this proposed difference in gene expression regulation between natural variants and laboratory strains of S. cerevisiae, we evaluated the karyotype and gene expression studies performed by Hose et al. on wild S. cerevisiae strains. In contrast to the results of Hose et al., our reexamination of their data revealed a tight correlation between gene copy number and gene expression. We conclude that widespread dosage compensation occurs neither in laboratory strains nor in natural variants of S. cerevisiae.
منابع مشابه
Further support for aneuploidy tolerance in wild yeast and effects of dosage compensation on gene copy-number evolution.
In our prior work by Hose et al., we performed a genome-sequencing survey and reported that aneuploidy was frequently observed in wild strains of S. cerevisiae. We also profiled transcriptome abundance in naturally aneuploid isolates compared to isogenic euploid controls and found that 10-30% of amplified genes, depending on the strain and affected chromosome, show lower-than-expected expressio...
متن کاملEffect of RP51 gene dosage alterations on ribosome synthesis in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae ribosomal protein rp51 is encoded by two interchangeable genes, RP51A and RP51B. We altered the RP51 gene dose by creating deletions of the RP51A or RP51B genes or both. Deletions of both genes led to spore inviability, indicating that rp51 is an essential ribosomal protein. From single deletion studies in haploid cells, we concluded that there was no intergenic dos...
متن کاملA New Strategy for Simultaneous Compensation of Instantaneous Reactive Power and Harmonics of Non-Linear Loads (RESEARCH NOTE)
This paper presents a new control method for simultaneous compensation of instantaneous reactive power and current harmonics by parallel active filters (PAFs). Reference compensating currents of PAF are calculated using the subtraction of instantaneous power from its average value. In this way, it is possible omitting high/low pass filter(s) from the control circuit of PAF, which usually result...
متن کاملP-18: Protective Effect of Selenium- Enriched Saccharomyces Cerevisiae Cytoplasm and Cell Wall on Chronic Immobilization Stress-Induced Damages in Testis; Evidence for Apoptosis
Background Previous reports showed that immobilization stress (IMS) results in severe damages at spermatogenesis level. Present study was performed in order to evaluate the protective effect of selenium-enriched yeast fragments on IMS-induced derangements. MaterialsAndMethods For this purpose, 42 mature male Wister rats were assigned into 6 groups (7 rats in each group) including; control, stre...
متن کاملPDC Control of Time Delay Fuzzy T-S modeled HIV-1 System through Drug Dosage
This paper proposes a Time Delay nonlinear dynamic model of HIV-1 (Human Immunodeficiency Virus type 1), introducing the drug consumption efficiencies as the controlling input for the model. The paper also represents the fuzzy T-S representation and the corresponding Fuzzy T-S controller. The controller parameters are tuned using LMIs (Linear Matrix Inequalities). The main focus is on the stabi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- eLife
دوره 5 شماره
صفحات -
تاریخ انتشار 2016